Lysyl Endopeptidase®, MS Grade

for Proteome Research.

  1. Storage Condition : Keep at -20 degrees C.
  2. CAS RN® : 123175-82-6
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Description

This product is for research use only. Do not administer it to human.

[BIOCHEMISTRY]

For Proteome Research!

Among the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modifications can be obtained.Lysyl Endopeptidase, Mass Spectrometry Grade is a freeze dried product that retained sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.

Additional information

Catalog No.

121-05063, 125-05061

Pack Size

20ug, 20ug x5

  1. High specificity and efficiency of protein digestion allow for easy database searches by peptide mass.
  2. Improved cleavage at lysine residue and increase in the number of peptides are obtained by combination with trypsin.
  3. Packed in very small quantities according to the amounts used so that sufficient activity for in-gel digestion may be retained.

Comparison of In-gel Digestion using Trypsin (Tp), Lysl Endopeptidase (Lep) and Lep Combined with Tp (Lep + Tp)

BSA band (100 ng) resolved by SDS-PAGE was in-gel digested with Tp, Lep and Lep + Tp and analyzed by MALDI-TOFMAS. The figure shows the individual mass spectra