Lysyl Endopeptidase®, MS Grade
for Proteome Research.
- Storage Condition : Keep at -20 degrees C.
- CAS RN® : 123175-82-6
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Description
This product is for research use only. Do not administer it to human.
[BIOCHEMISTRY]
For Proteome Research!
Among the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modifications can be obtained.Lysyl Endopeptidase, Mass Spectrometry Grade is a freeze dried product that retained sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.
Additional information
| Catalog No. | 121-05063, 125-05061 |
|---|---|
| Pack Size | 20ug, 20ug x5 |
- High specificity and efficiency of protein digestion allow for easy database searches by peptide mass.
- Improved cleavage at lysine residue and increase in the number of peptides are obtained by combination with trypsin.
- Packed in very small quantities according to the amounts used so that sufficient activity for in-gel digestion may be retained.
Comparison of In-gel Digestion using Trypsin (Tp), Lysl Endopeptidase (Lep) and Lep Combined with Tp (Lep + Tp)
BSA band (100 ng) resolved by SDS-PAGE was in-gel digested with Tp, Lep and Lep + Tp and analyzed by MALDI-TOFMAS. The figure shows the individual mass spectra







