NanoShield™ Trap Columns
NanoShield™ Trap Columns
NanoShield™ Trap Columns

NanoShield™ 5×100 NZ C18 trap column 3-Pack

5 cm x 100 μm C18 UHPLC trap column with 2 x nanoZero® fittings compatible with nanoViper™

Part numberNS1-50100C18-3NZ-3PK
Samples per day10 – 300
GradientsUse standard Aurora Series® analytical gradients for elution
ModeReverse-flush
Maximum loading capacity1 ug
Suggested loading capacity200 ng

Description

Chromatographic trap columns are essential in proteomic UHPLC–MS workflows, improving analyte focusing and enabling rapid sample loading with in-line sample clean-up to protect analytical columns from blockages and contamination while reducing ion suppression. These benefits extend column lifetime and reduce maintenance, supporting robust, high-throughput, and reproducible proteomic analyses. Despite these advantages, trap columns traditionally sacrifice peptide and protein identifications compared to direct injection. The NanoShield™ C18 5 cm × 100 μm trap column eliminates this trade-off – delivering direct injection-level performance; providing superior peptide identifications, peak quality, and hydrophilic peptide coverage compared to other traps.

Product benefitsIdeal for
1. Next to no peptide ID loss

2. Extends column life, reduces maintenance costs

3. Improved reproducibility across thousands of injections

4. Enables hundreds of samples per day

1. Fast sample loading

2. Reversed-flush workflows

3. Bottom-up discovery workflows

A perfectly paired trap for Aurora Series columns.

A 100 ng HeLa tryptic digest was analysed using a reverse-flush trap-elute method compared to direct injection across two workflows: a 37-minute gradient on the Aurora® Ultimate™ CSI (25 cm x 75 μm) column and a 14-minute gradient on the Aurora® Rapid™ CSI (8 cm × 75 μm) column, both analysed on a Bruker nanoElute 2 UHPLC coupled to a Bruker timsTOF HT mass spectrometer.

The NanoShield™ trap column (5 cm x 100 μm, 3 μm C18 particles) demonstrated near-direct injection performance across all metrics while substantially outperforming a competitor C18 trap cartridge (5 mm x 300 μm, 5 μm C18 particles). NanoShield™ showed less than 1% reduction in protein identifications and a 1–3% reduction in precursor identifications compared to direct injection across both columns.

Peak quality metrics remained similarly robust, with marginal increases in FWHM and minimal changes to peak shape ratios compared to direct injection, while maintaining significantly superior performance relative to the competitor trap.

SpecificationDescription
Column formatTrap/guard column
Column typeReversed-phase
Trap column orientationReverse flush
For use withUHPLC
Length5 cm
Inner diameter100 µm
Pore size120 Å
Pressure rating>1700 bar
Particle size3 µm
pH stability1–8
Stationary phaseC18
Suggested loading capacity200 ng
Max loading capacity1 µg
Suggested loading pressure< 1000 bar

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