ISOSPIN Viral RNA Kit

Features
– Viral RNA can be purified in approximately 30 minutes
– Highly sensitive detection possible using PCR etc.
– High operability with large-capacity spin columns (column volume up to 970 μl)

Request to COA Click Here

Description
Additional information

Description

ISOSPIN Viral RNA kit is designed for the rapid preparation of viral RNA from fluid biological samples, such as nasopharyngeal and oropharyngeal swab washes, serum, saliva, and sputum. With the ISOSPIN Viral RNA method, RNA viruses are lysed quickly by NIRV Extraction Buffer and Proteinase K. Ethanol is added to the lysate and the lysate and ethanol create appropriate conditions for binding of RNA to the silica-based membrane of the Spin Column. Impurities are removed by washing with two kinds of Wash Buffers. The RNA is eluted in RNase-free water. The Spin Column of the ISOSPIN Viral RNA kit enables a high loading capacity. ISOSPIN Viral RNA kit allows purification of pure RNA in 30 minutes without phenol/chloroform extraction or alcohol precipitation.

Advantages

Designed to purify viral RNA in 30 min
High loading capacity of Spin Column and good operability
Ideal for sensitive applications such as RT-qPCR

Works for a wide variety of viruses, including:

Coronavirus (SARS-CoV-2)
Poliovirus
Hepatitis E virus (HEV)
Severe febrile thrombocytopenia syndrome virus (SFTSV)

**ISOSPIN Viral RNA (for 50 times)**
Components	capacity	keep	remarks
Proteinase K (20 mg/ml)	1 ml x 1 bottle	-20°C
NIRV Extraction Buffer	18 ml x 1 bottle	room temperature	Crystals may precipitate at low temperatures. In this case, heat to approximately 37-65°C, shake well, and use after complete dissolution.
NIRV Wash1 Buffer	30 ml x 1 bottle	room temperature	Contains ethanol
NIRV Wash2 Buffer	30 ml x 1 bottle	room temperature	Contains ethanol
ddWater (RNase-free)	1 ml x 3 bottles	room temperature
Spin Column	50 pieces x 1 bag	room temperature	Upper part: Column, Lower part: Collection Tube
Collection Tube	50 pieces x 2 bags	room temperature	Collection Tube (single item)