DNA Extractor® Kit
DNA Extractor® Kit
DNA Extractor® Kit

for Genetic Research
Storage Condition : Keep at RT.

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Description

This is for research use only. Do not administer it to human. Molecular Biology-DNA Extraction Kits. Detects and quantitates contaminant DNA in serum and residual DNA in biopharmaceuticals. Employs a new extraction procedure for DNA purification from a single tube. This procedure, using Sodium Iodide (NaI) as a chaotropic agent, realizes DNA isolation of both high quality and high recovery from biological fluids without the use of phenol or chloroform. Complex and laborious manipulations are avoided when using this kit. In addition, this kit has a modified application for use with Molecular Devices’ Threshold(TM) System.

PRINCIPLE of DNA EXTRACTION: A high concentration of chaotropic reagent, NaI, and an anionic detergent participate in solubilization of the proteins and lipids contained in biological samples. After addition of isopropanol to the mixture, nucleic acids are co-precipitated with polysaccharide glycogen as a carrier, while other components remain soluble in the solution phase.

50 tests

Sodium Iodide Solution1 x 26 mL
Sodium N-Lauryl Sarcosinate Solution1 x 1.2 mL
Washing Solution (A)1 x 42 mL
Washing Solution (B)2 x 40 mL
Glycogen Solution1 x 0.1 mL

Additional information

Catalog No.

295-50201

Pack Size

50 Tests

Spike and recovery test of CHO cell-derived DNA

Examined the recovery rate of CHO cell-derived DNA from spiked culture supernatant to evaluate the extraction efficiency.

Methods
  1. Added 10 fg to 1 ng of CHO cell-derived DNA to culture supernatant of PANC-1 cells.
  2. Extracted DNA from the supernatant with this kit following the manual.
    -Protocol: Protocol #2
    -Pretreatment: None
  3. Conducted qPCR and measured the Cq value of the extracted DNA. The same measurement was conducted for purified water spiked with CHO cell-derived DNA without DNA extraction, this was used as standard condition.
  4. Create a standard curve using the mean Cq values of the standard condition, and calculated the recovery rate.
Samples
  1. Purified water containing CHO cell-derived DNA (no DNA extraction): Standard conditions
  2. DNA extracted from the culture supernatants of PANC-1 cells containing CHO cell-derived DNA by this kit: Culture supernatants
qPCR reagents
  • GeneAce SYBR® qPCR Mix α No ROX (Nippon Gene#319-07703)
  • Optical Flat 8–Cap Strips for 0.2 mL tube strips/Plates (BIO-RAD#TCS0803)
  • Hard-Shell® Thin-wall 96-well PCR plates (BIO-RAD#HSP9601)

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