BCA Estimation Kit with Protein Standard
BCA Estimation Kit with Protein Standard
BCA Estimation Kit with Protein Standard
Accurate protein quantification is essential in life science research, from enzyme activity studies and protein–protein interaction assays to sample preparation for advanced downstream analyses.

Description

Accurate protein quantification is essential in life science research, from enzyme activity studies and protein–protein interaction assays to sample preparation for advanced downstream analyses. The BCA Protein Estimation Kit provides a dependable solution, delivering precise, reproducible results across a wide range of biological samples, including cell lysates, serum, plasma, and urine.

Using the well-established bicinchoninic acid (BCA) chemistry, the assay produces a stable purple color when protein–copper complexes are formed under alkaline conditions. The intensity of this color, measurable at 562 nm, directly correlates with protein concentration, ensuring consistent and quantitative assessment.

This kit is supplied with following components:

ReagentCatalog No.Storage
APX-RGA-101Room Temperature
BPX-RGB-101
Protein StandardPX-PS-1012-8℃
SPECIFICATION
SpecificationDescription
MethodBCA (Bicinchoninic Acid) Protein Assay
Reference ProteinBSA (Bovine Serum Albumin), 2 mg/mL
Intended UseSolution-based protein detection, absorbance measurement
InstrumentsSpectrophotometer, Microplate Reader
Components10 × 1 mL glass ampoules
CategoryProtein Quantitation Assay
Pack Size1 Kit (250 ml) and (500ml)
Target RangeGeneral protein quantification (not target-specific)
Throughput~250 cuvette assays and ~500 cuvette assays
ReadoutColorimetric (absorbance at 562 nm)
Packaging UnitEach

Additional information

Catalog No.

PX-PROTQ-101-250ML, PX-PROTQ-101-500ML

Pack Size

250 mL, 500 mL

Key Advantages

Broad Compatibility – Works effectively in the presence of ionic and non-ionic detergents, making it ideal for lysates and extraction buffers.

High Accuracy – Less affected by protein-to-protein variation than dye-binding assays such as Bradford.

Convenient & Efficient – Simple protocol with minimal hands-on time; results in under 30 minutes.

Flexible Format – Sufficient for ~2,500 microplate reactions or 500 cuvette assays.

Precautions and Disclaimer

For research and development use only. This product is not intended for drug, household, or any other unauthorized applications

Key Documents

  1. COA (Certificate of Analysis)
CategoryProduct CodeProduct Name
Safety Data Sheet (SDS)PX-PS-101Protein standard (BSA)
PX-RGA-101Reagent A
PX-RGB-101Reagent B

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